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Activation of VMN Adcyap1 cells increases BAT temperature. We administered an AAV that introduces hM3dq in Adcyap1 cells in the VMN (A) and measured interscapular BAT (iBAT) temperature in response to both clozapine-n-oxide (CNO) and Saline (vehicle control) (B). Data are expressed as mean ± SEM. Results were analyzed by 2-way repeated-measures ANOVA. Open circles and bars refer to Saline control and closed circles and bars refer to CNO. n = 5, *P < 0.05.
