Figure 4.

Adcyap1 ^MBH KO mice have impaired energy expenditure measured by indirect calorimetry. At 4 weeks postinjection, we measured body composition via Echo MRI (A-B). At 5 weeks, we weighed interscapular BAT (iBAT) tissue (C). Food intake was collected in the metabolic chambers (D). VO2 and VCO2 were analyzed by normalizing lean mass (E, F). Respiratory exchange ratio, ambulatory activity, fat oxidation, and glucose oxidation were also measured in the metabolic chambers (I, K, M, O). In addition to data collected every 40 minutes, average values separately by light and dark cycle are also included (F, H, J, L, N, P). Data are expressed as mean ± SEM. Results were analyzed by 2-way ANOVA with Fisher least significant difference (LSD) post hoc tests in F, H, J, L, N, P, and repeated-measures ANOVA with Fisher LSD post hoc tests in D, F, H, J, L, N. Results were measured by Student t test in A-D. Open circles and bars refer to GFP control and closed circles and bars refer to Cre. n = 5 (GFP), 7 (cre), *P < 0.05.