Figure 5.

Adcyap1 ^MBH KO mice are hyperglycemic and hyperinsulinemic, but not insulin resistant. In addition to body weight and food intake-related measures, we determined if glycemic factors were also out of balance. We measured glucose (A), insulin (B), glucagon (C), and corticosterone (D) at both fed state and post-overnight fast. All of these tests were performed during the light cycle. We also performed glucose (2 g/kg) tolerance test (E) and insulin (0.6 U/kg) tolerance test (F). We also measured corticosterone (G) and glucagon (H) levels 30 minutes following insulin injection. Data are expressed as mean ± SEM. Results were analyzed by 2-way ANOVA with Fisher LSD post hoc tests in A-D and repeated-measures ANOVA with Fisher least significant difference post hoc tests in E-F. Results in G-H were analyzed by Student t test. Open circles and bars refer to GFP control and closed circles and bars refer to Cre. n = 16 (GFP), 22 (cre), *P < 0.05.