Figure 7.

Adcyap1 ^MBH KO mice do not have impaired glucose clearance prior to obesity. Before mice were obese, glucose clamps were performed during the light cycle in a cohort of male mice to determine the direct function of the peptide versus effects secondary to obesity. We measured body weight (A), fat mass (B), and lean mass (C) prior to clamp. Glucose was clamped at 150 mg/dL (D). We measured glucose infusion rate (E). In addition, we measured glucose disposal (F) and hepatic glucose production (G) both before and during the clamp. Lastly, we measured the uptake of radiolabeled 2-deoxyglucose in the muscle (H), brown adipose tissue (I), visceral fat (J), subcutaneous fat (K), and heart (L) after the clamp. Data are expressed as mean ± SEM. Results were analyzed by Student t test in A-C, H-K, repeated-measures ANOVA with Fisher least significant difference (LSD) post hoc tests in D-E, and 2-way ANOVA with Fisher LSD post hoc tests in F-G. Open circles and bars refer to GFP control and closed squares and bars refer to Cre. n = 5 (GFP), 7 (cre).