Skip to main content
. Author manuscript; available in PMC: 2021 Oct 28.
Published in final edited form as: J Am Chem Soc. 2020 Oct 14;142(43):18449–18459. doi: 10.1021/jacs.0c06877

Figure 2.

Figure 2.

Enrichment of PDMs with DIMEN. (a) LC-HRMS chromatograms for a typical enrichment experiment. Peaks of highly abundant nontarget metabolites (red) are absent following enrichment and remain in the flow-through (intensities adjusted for sample volumes). (b) MS/MS of PDMs in positive mode produces a reporter-ion at m/z 100.0757. (c) Extracted-ion-chromatogram (EIC) for the reporter ion m/z 100.076 of a DIMEN-enriched sample, showing peaks representing known metabolic transformations (annotated in blue), novel transformations (examples annotated red), and unmodified probe and nonbiological cleavage products (annotated in green). See Figure S1 for structures.