Table 1. Primers used in referred studies.

Gene	Exon	Sequence
BRAF	15	F: 5′-biotin-GCTTGCTCTGATAGGAAAATGA-3′
		R: 5′-GACAACTGTTCAAACTGATGGG-3′
		S: 5′-CCACTCCATCGAGATTT-3
NRAS	1	F: 5′-GGTGTGAAATGACTGAGTACAAACTGG-3′
		R: 5′-biotin-CATATTCATCTACAAAGTGGTT CTGGA-3′
		S: 5′-CAAACTGGTGGTGGTTGGAG-3′
	2	F: 5′-GATTCTTACAGAAAACAAGTGGTTAT AGAT-3′
		R: 5′-biotin-GCAAATACACAGAGGAAGCCT TCG-3′
		S: S: 5′-GACATACTGGATACAGCTGG-3′
TERT promoter		F: 5′-CCCACGTGCGCAGCAGGAC-3′
		R: 5′-Biotin-CTCCCAGTGGATTCGCGGGC-3′
		S: 5′-AGGGGCTGGGAGGGC
KIT	17	F: 5′-AAAGATTTGTGATTTTGGTCTAGC-3′
		R: 5′-GAAACTAAAAATCCTTTGCA-3′
GAPDH	2	F: 5′-CACTAGGCGCTCACTGTTCT-3′
		R: 5′-GCGAACTCACCCGTTG-3′
PTE promoter		F: 5′-GGATGTGGGTGTTTGTGTAATTA-3′
		R: 5′-biotin-AATTCCCACTCCCCAATAATAAC-3′
		S: 5′-TTTGTGTAATTAGTTTTTTA-3′

F: forward primer, R: reverse primer, S: sequencing primer, GAPDH: glyceraldehyde-3-phosphate dehydrogenase.