Fig. 6. Standard reference material (SRM) 1649b exposure reduced the mRNA and protein expression levels of FLG, INV, LOR, CLDN-1, and ZO-1 in both control and oxazolone (OXA)-induced atopic dermatitis (AD) groups. However, the decrease in the expression of the stratum corneum-related proteins and tight junction proteins after SRM exposure was comparable between the two groups. The mRNA and protein levels of skin barrier-related proteins were analyzed using (A, B) real-time quantitative PCR and (C) western blotting, respectively. The relative mRNA and protein expression levels in the AD-like mouse model are shown. These levels were normalized to the mRNA expression levels of β-actin. Values are presented as mean±standard deviation of two independent experiments (n=2). FLG: filaggrin, INV: involucrin, LOR: loricrin, CLDN-1: claudin-1, 1649b group: SRM 1649b-exposed control group, AD+1649b group: SRM 1649b-exposed OXA-induced AD group, AD group: OXA-induced AD group. ^*p<0.05.