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. Author manuscript; available in PMC: 2021 Feb 10.
Published in final edited form as: Sci Transl Med. 2020 Jan 29;12(528):eaax6795. doi: 10.1126/scitranslmed.aax6795

Fig. 3. Changes in activation state, proliferative status, and phenotypic marker expression following repeated activation of CD4+ T-cell subsets.

Fig. 3.

(A) Activation status of CD4+ T-cells throughout the MSVOA averaged across 4 representative individuals as percent of cells expressing CD25 and/or CD69. (B) CFSE dilution after first stimulation. Cells from each subset were stained with CFSE and activated on day 0, then analyzed by flow cytometry at days 4 and 9. (C) CD45RO expression on TN from representative individual. 91.6% of TN became CD45RO+ by day 4 after initial stimulation (Fig. S1A). (D) Percent of cells from each subset culture expressing CCR7 and CD27 at the indicated timepoints. Arrows denote stimulation timepoints.