Table 1.
Summary of scRNAseq Studies Performed on Non-Immune Stromal Cells from White Adipose Tissue.
| Reference | WAT Depot | Sex | Age | Strain | Stimulus | scRNA Cell Sorting Technique | ASC Populations (identified by authors) | Proposed ASC Nomenclature | Cell Sorting Strategy (Functional Assays)** |
|---|---|---|---|---|---|---|---|---|---|
| Burl et al. (64) | eWAT | M | 8–10 Wk | C57BL6/J | SHAM or 3 days CL-316,243 (0.75 nmol/hr) |
Lineage Negative (CD45-, CD45R-, CD11b-, Gr-1 (Ly-6G/C)-, 7–4-, and Ter-119-) |
ASC1 ASC2 |
ASC1a ASC2 |
ND |
| Hepler et al. (104) | eWAT | M | 8 Wk | PdgfrbrtTA (C57BL6/J) | None | “Muralchaser” tdTomato- mGFP+ SVC |
APC FIPs |
ASC1a ASC2 |
Pdgfrβ+Ly6C-CD9-Pdgfrβ+Ly6C+ |
| Burl et al. (64) | iWAT | M | 8–10 Wk | C57BL6/J | SHAM or 3 days CL-316,243 (0.75 nmol/hr) |
Lineage Negative (CD45-, CD45R-, CD11b-, Gr-1 (Ly-6G/C)-, 7–4-, and Ter-119-) |
ASC1 ASC1 ASC2 |
ASC1a ASC1b ASC2 |
ND |
| Merrick et al. (102) | iWAT | M/F | PND12 | C57BL6/J | None | Lineage Negative (CD45-) |
Group2 Group3 Group1 |
ASC1a ASC1b ASC2 |
Icam+CD142- CD142+ DPP4+CD142- |
| Merrick et al. (102) | iWAT | M | 10 Wk | 129S6/SvEv | None | Lineage Negative (CD45-) |
Group2 Group3 Group1 |
ASC1a ASC1b ASC2 |
Icam+CD142- CD142+ DPP4+CD142- |
| Schwalie et al. (103) | iWAT | M/F | 8 Wk | Tg(Pref1-CreER)426 Biat (Pref1-CreER) | None | Lineage Negative (CD31−CD45−Ter119−) | G2 (P2*) G3 (P3*) G1/G4 (P1*) |
ASC1a ASC1b ASC2 |
Vap1+Adam12+ CD142+Abcg1+ CD55+IL13ra+ |
*
ASC groups in parenthesis refer to the author’s nomenclature for scRNA groups identified using the Fluidigm C1.
**
Cell sorting strategy for Hepler et al. (104) and Merrick et al. (102) were selection markers after depleting CD45+CD31+ stromal vascular cells. See text for details of additional sorting strategies used by Schwalie et al. (103).
Abbr: Adipose stem cells (ASC), adipose progenitor cells (APC), fibro-inflammatory progenitors (FIP), stromal vascular cells (SVC).