Skip to main content
. Author manuscript; available in PMC: 2023 Sep 5.
Published in final edited form as: ACS Synth Biol. 2021 Feb 3;10(2):379–390. doi: 10.1021/acssynbio.0c00566

Figure 2.

Figure 2.

Employing sdAb-APEX2 fusions to recognize recombinant or viral N protein within mammalian cells. (A) APEX2 fusion proteins of the WuN1 and WuN3 sdAb used as probes at 100 nM for recombinant N protein or β-gal transiently expressed in HEK293T cells 24 h post transfection, or SARS-CoV-2 infected or uninfected Vero cells 24 h post infection. Signal development used either Amplex™ UltraRed and fluorescent microscopy or DAB and bright field (10 x magnification). (B) 150x magnification of the SARS-CoV-2 infected cells developed with fluorescent or colorimetric substrate.