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. 2021 Feb 10;16(2):e0245179. doi: 10.1371/journal.pone.0245179

Fig 2. MG53 gene knock-down does not affect insulin signaling in differentiated C2C12 cells.

Fig 2

C2C12 cells were transfected with small interfering RNA targeting MG53 or a non-silencing control siRNA (10nM) using Lipofectamine RNAi max. 48h post-transfection, MG53 mRNA (Panel A) and protein levels (Panel B) were assessed by qPCR and western blot analysis, respectively. Vinculin was used as a loading control (Panel B). Insulin response (10 or 100nM for 10 minutes) was assessed by monitoring Akt phosphorylation on Serine 473 (Panel C). Finally, glucose uptake was measured using 2DG-glucose in response to insulin treatment (100nM) (Panel D). Data are expressed as percentage of control (Mock-transfected cells).