Fig 4. MG53 over-expression does not affect insulin response in HEK293 cells.

HEK293 cells were transfected with a MG53 expression plasmid or GFP as control. 24h (left panel) or 48h later (right panel), cells are exposed to increasing concentrations of insulin (10 and 100nM) for 15 minutes. Insulin response was then evaluated using phospho Akt levels.