Fig 5. mg53 gene deficiency does not affect high fat diet-induced obesity and glucose intolerance.

Panel A: targeting strategy to engineer mg53 -/- mice. Panel B: western blot analysis of MG53 expression in both wild type and KO mice. MW: molecular weight marker. 10μg of total protein lysate from human skeletal muscle was used as positive control. Panel C: Total body weight in both males and females wild-type and mg53 KO mice after 16 weeks of high fat diet. Panel D: An oral GTT was performed in both male and female mice (wild-type vs. KO mice) after 16 weeks of high fat diet.