Skip to main content
. 2021 Feb 10;16(2):e0246918. doi: 10.1371/journal.pone.0246918

Fig 5. Independent protein expression by two plasmids co-existing in A. baumannii.

Fig 5

A. The coding sequence of the green fluorescence protein (gfp) was inserted into pJL01 and the resulting plasmid was introduced into A. baumannii strain ATCC_17978. The expression of Flag-GFP was detected as described in Fig 3. B. Cultures of A. baumannii strain ATCC_17978(pJL04::LacZ, pJL01::GFP) were induced by arabinose, IPTG or both and the protein samples resolved by SDS-PAGE were detected by immunoblotting with the Flag-specific antibody. The signals of a band with a molecular weight of approximately 50 kDa detected nonspecifically by the antibody were used as a loading control. Results shown are one representative from at least three independent experiments with similar results.