Table 1. Bacterial strains and plasmids used in this study.
| Strains or plasmids | Relevant features | Source |
|---|---|---|
| A. baumannii | ||
| ATCC 17978 | Type strain | [15] |
| ATCC 17978StrpR | StrepR, a derivative of ATCC 17978 resistant to streptomycin | This study |
| 17978ΔhisD | Deletion mutant of the histidinol dehydrogenase gene (hisD, A1S_0687) | This study |
| E. coli | ||
| DH5 | E. coli strain for molecular cloning | [50] |
| BL21(DE3) | E. coli strain for protein expression from the T7 RNA polymerase | [51] |
| S17-1 | E. coli strain for biparental conjugation, recA pro hsdR, RP4-Tc::Mu-Km::Tn7 | [31] |
| MT607(pRK600) | CmR, E. coli helper strain for triparental conjugation, | [32] |
| Plasmids | Source | |
| pDSK519 | KmR, an IncQ plasmid derived from RSF1010 | [27] |
| pBAD22 | AmpR, A ColE1 plasmid carrying the PBAD promoter and the rrnB T1T2 transcriptional terminators | [22] |
| pBAD22Bam-1 | A derivative of pBAD22 lacking a BamHI site in the end of the PBAD promoter | This study |
| peGFPC1 | KmR, source for the gfp gene | Clontech |
| pJY100 | GmR, a synthetic plasmid harboring the lacIQ gene, the PTAC promoter and 5 copies of the lac operator | This study |
| pVRL1 | GmR, shuttle plasmid for A. baumannii | [21] |
| pVRL1StuI | GmR, a derivative of pVRL1 with a StuI restriction site | This study |
| pJL01 | KmR, a pDSK519-based plasmid harboring PBAD | This study |
| pJL02 | KmR, a pDSK519-based plasmid harboring PTAC | This study |
| pJL03 | GmR, a pVRL1-based plasmid harboring PBAD | This study |
| pJL04 | GmR, a pVRL1-based plasmid harboring PTAC | This study |
| pSR47s | KmR, sacB, a R6K plasmid for gene deletion | [35] |
| pSR47sΔhisD | KmR, a pSR47s derivative for deleting hisD (A1S_0687) in A. baumannii | This study |
| pET28a::ICDH | KmR, a plasmid for the production of the ICDH protein of A. baumannii in E. coli | This study |
| pJL01::LacZ | KmR, the E. coli lacZ gene cloned in pJL01 | This study |
| pJL02::LacZ | KmR, the E. coli lacZ gene cloned in pJL02 | This study |
| pJL03::LacZ | GmR, the E. coli lacZ gene cloned in pJL03 | This study |
| pJL04::LacZ | GmR, the E. coli lacZ gene cloned in pJL04 | This study |
| pJL01::HisD | KmR, the A. baumannii hisD gene cloned in pJL01 | This study |
| pJL02::HisD | KmR, the A. baumannii hisD gene cloned in pJL02 | This study |
| pJL03::HisD | GmR, the A. baumannii hisD gene cloned in pJL03 | This study |
| pJL04::HisD | GmR, the A. baumannii hisD gene cloned in pJL04 | This study |
| pJL01::eGFP | KmR, the enhanced gfp gene cloned in pJL01 | This study |
Note: Cm, chloramphenicol; Km, kanamycin, Gm, gentamicin; Strep, streptomycin.