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. 2021 Jan 29;17(1):e1009285. doi: 10.1371/journal.pgen.1009285

Fig 6. Orthogonal CRISPR screen for modifiers of LDL uptake by HepG2 cells.

Fig 6

(A-F) Volcano plots displaying MAGeCK gene level enrichment score and log2 fold change for each gene tested in the secondary gRNA library, under lipoprotein-rich (A) or lipoprotein-depleted (D) cultured conditions, with genes identified with FDR<5% displayed in red. Venn diagrams demonstrating the overlap between HuH7 and HepG2 cells for positive (B, E) and negative (C, F) regulators of LDL uptake under lipoprotein-rich (B-C) or lipoprotein-depleted (E-F) culture conditions. (G) Positive regulators of LDL uptake in HuH7 cells under lipoprotein-depleted conditions were grouped by quartile and the proportion in each group that also influenced LDL uptake in HepG2 cells is displayed. (H) The effect size in lipoprotein-depleted conditions for gene-level gRNA enrichment in each cell type is plotted for genes showing a functional role in either cell type.