Fig. 1.

Synphilin-1 increases AMPK phosphorylation in flies. (A-C) The homogenates from adult non-transgenic and Elav-GAL4;UAS-SP1 transgenic 10-day-old flies with or without UAS-AMPK RNAi (25931, 32371 and 35137) were subjected to western blot analysis (A, representative blots) using anti-phospho-AMPK, anti-AMPK, anti-synphilin-1 and anti-actin antibodies. In each experiment, three fly heads were pooled together for one sample. There were six samples in each experimental group for western blot analysis. The quantification of total (B, *P<0.05 versus NTg group) and phosphorylated (C, *P<0.05 represents significance between groups as indicated) AMPK levels was normalized to actin. Data are mean±s.e.m. and were analyzed by ANOVA followed by Tukey's post-hoc test.