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. 2021 Feb 10;7(7):eabd8217. doi: 10.1126/sciadv.abd8217

Fig. 2. Angiogenic activity of various polymers.

Fig. 2

(A) Characterization of the polymers by zeta potential analysis and elemental analysis. (B and C) Representative angiographic images (B) and quantification of relative blood vessel volume (BVV/TV) (C) in implants at day 7, respectively (n = 3). Scale bars, 1 mm. (D) H&E staining of implants at day 7 in mice ischemic hind limb. Scale bars, 100 μm. Insets are macroscopic views of the indicated implants. Photo credit: Y.Y., East China University of Science and Technology. (E to H) Representative immunostained images (E and G) with quantification of CD31hiEmcnhi (Type H) endothelium (F) and α-SMA+ arteries (H) in implants on day 7 after implantation in ischemic limb (n = 5). Scale bars, 100 μm. (I) Measurement of ex vivo anticoagulation; 2 μg of PSS, heparin, or SCS was added into 200 μl of blood plasma from rabbit at 37°C (n = 6). Data are means ± SD. ***P < 0.05 and ****P < 0.001; ns, not significant [(C), (F), and (H), one-way ANOVA compared to SCS GS with Dunnett’s post hoc test; (I), one-way ANOVA compared to heparin with Dunnett’s post hoc test].