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L-Cysteine, copper ions bound to albumin (Cu-ALB) and S-[15N]nitrosoalbumin (Cu-S15NALB) are required for the formation of 15 N-nitrite from S15NALB. The initial concentrations were 13 µM for S15NALB and 15 µM for Cu-S15NALB. The concentration of free L-cysteine added to the samples (+Cys) was 100 µM. The concentration of free L-cysteine in the samples non-spiked with L-cysteine (-Cys) is unknown. The protein concentration in ALB, Cu-ALB, S15NALB and Cu-S15NALB was about 300 µM.
