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. 2021 Jan 24;36(1):480–490. doi: 10.1080/14756366.2021.1876686

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Chromatograms of the reduced and alkylated pTTI by reverse-phase high-performance liquid chromatography treated with digestive enzymes with degree sequencing for mass spectrometry. (A) Chromatographic profile of pTTI digested with trypsin in a C2/C18 Pharmacia Biotech µRCP analytical column. (B) Chromatographic profile of pTTI digested with GluC in a C2/C18 Pharmacia Biotech µRCP analytical column. pTTI: Purified trypsin inhibitor from tamarind seeds.