FIG. 5.

Alcohol self-administration, but not TBI, alters endocannabinoid system protein expression in the BLA of female rats 11 days post-injury. BLA collected from animals in Expt. 1 were probed for eCB system proteins via western blotting 11 days post-TBI. Alcohol-drinking animals showed an increase in DAGLβ expression (A), with a trend toward a decrease in the expression of DAGLα (B), and no change in MAGL (C), or CB1R (D). Data were analyzed via two-way ANOVA, *p < 0.05 main effect of alcohol self-administration, n = 5–9/group. Data are expressed as mean ± SEM. ANOVA, analysis of variance; BLA, basolateral amygdala; CB1R, cannabinoid receptor 1; DAGL, diacylglycerol lipase; eCB, endocannabinoids; MAGL, monoacylglycerol lipase; SEM, standard error of the mean; TBI, traumatic brain injury.