. 2021 Feb 3;2021:4853632. doi: 10.1155/2021/4853632

Table 1.

Primers used in comparison of candidate reference genes in Spinach.

Gene name¹	Gene ID²	Sequence of primer (forward/reverse)	Size of PCR product (bp)	Amplification efficiency (%)	Correlation coefficient (R²)
18S rRNA	Spo14194	GATTCCGACGAACAACTGCG	141	98.96	0.998
18S rRNA	Spo14194	AAGTAACATCCGCCGATCCC	141	98.96	0.998
Actin	Spo23599	TGTTCACGACATCAGCCGAA	138	99.36	0.999
Actin	Spo23599	CGTCGGGTAGCTCGTAGTTC	138	99.36	0.999
ARF	Spo09845	CCGATAAGCTTGGCCTCCAT	125	92.53	0.997
ARF	Spo09845	AGCCTTGCTAGCGATGTTGT	125	92.53	0.997
COX	Spo27102	AGGTTGCTCATGCTGTCTTGA	168	94.85	0.991
COX	Spo27102	CAACGACACTGATCTGGCCT	168	94.85	0.991
CYP	Spo15438	TCCTTTCCATGGCCAATGCT	132	93.11	0.999
CYP	Spo15438	CCCTAACAACGTCCATGCCT	132	93.11	0.999
EF1α	Spo03008	ACCTCTCAGGCTGATTGTGC	173	102.80	0.995
EF1α	Spo03008	GAGTACTTGGGAGTGGTGGC	173	102.80	0.995
GAPDH	Spo24687	GGCTGCCATCAAGGAGGAAT	129	93.56	0.999
GAPDH	Spo24687	GCAATTCCAGCCTTGGCATC	129	93.56	0.999
H3	Spo20638	AAGAAGCCTCACCGTTACCG	178	94.48	0.998
H3	Spo20638	CCTCCTGAAGGGCCAAAACA	178	94.48	0.998
RPL2	Spo08157	TTCTCGTCCGTCTCCCTTCT	198	101.18	0.997
RPL2	Spo08157	TACCCTCACCACCACCATGA	198	101.18	0.997
TUBα	Spo15071	TAATGCCGCTGTTGCTACCA	137	94.90	0.998
TUBα	Spo15071	CTCTCTGCACCTTGGCAAGA	137	94.90	0.998

¹Full names of these 10 genes are 18S ribosomal RNA (18S rRNA), actin, ADP ribosylation factor (ARF), cytochrome c oxidase (COX), cyclophilin (CYP), elongation factor 1-alpha (EF1α), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), histone H3 (H3), 50S ribosomal protein L2 (RPL2), and tubulin alpha chain (TUBα). ²These sequences and IDs were obtained from SpinachBase (http://www.spinachbase.org).