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. 2021 Feb 11;227:122154. doi: 10.1016/j.talanta.2021.122154

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Fig. 2 — LCR assay optimization. (A) The influence of salmon sperm DNA concentrations and PfAgo reaction times on PLCR of SARS-CoV-2. (B) The influence of salmon sperm DNA concentrations and PfAgo reactions times on PLCR of HPV11. (C) The normalized photoluminescent (PL) signal after PLCR for different ligation times of one cycle and different reaction times of PfAgo. (D) The normalized photoluminescent (PL) signal after PLCR linking two oligonucleotides with SplintR ligase or performing reverse transcription using RNA as a template. Data are presented as mean ± SD of three technical replicates.