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. 2021 Feb 11;227:122154. doi: 10.1016/j.talanta.2021.122154

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Fig. 3 — Analysis of the sensitivity of PLCR. (A) The normalized photoluminescent (PL) signal after PLCR for different concentrations of SARS-CoV-2. The LCR was carried out at 80 °C for 5 s, 40 °C for 1 min for 30 thermal cycles, followed with PfAgo digestion for 20 min. (B) The normalized photoluminescent (PL) signal after PLCR for different concentrations of HPV11. The LCR was carried out at 80 °C for 5 s, 40 °C for 1 min for 30 thermal cycles, followed with PfAgo digestion for 20 min. (C) Calibration plot of the normalized photoluminescent (PL) intensity versus logarithmical concentration of SARS-CoV-2 N gene (from 10 aM to 10 pM). (D) Calibration plot of the normalized photoluminescent (PL) intensity versus logarithmical concentration of HPV11E6. (from 10aM to 1pM). Data are presented as mean ± SD of three technical replicates. (P < 0.05; **, P < 0.01; ***, P < 0.001; ****).