Table 1.

HPLC mobile phase gradient program and detection conditions^*.

Chromatographic Conditions	Detector Conditions
Mobile phase: Water/Acetonitrile	0−13 min: Ex 270/Em 430nm
0−2 min: 50 % water -50 % ACN	13−19 min. Ex 270/Em 390nm
2−15 min: 100 % ACN, hold 8 min.	19−20.9 min. Ex 258/Em 430nm
23−25 min: back to initial condition	21−23 min: Ex 290/Em 430nm
Flow: 0.8 mL/min	23−25 min: Ex 270/Em 430nm

^*Analysis run time 25 min with 5 min post run re-equilibration time.