FIGURE 3.

ACLY promotes HCC cells proliferation, migration and invasion. We constructed HCC‐LM3 cells with ACLY overexpression, and HepG2 cells with ACLY knockdown. (A) Western blot was utilized to investigate ACLY expression in HCC‐LM3 and HepG2 cells after construction. The effects of knockdown or overexpression of ACLY on proliferation and clone formation of HepG2 and HCC‐LM3 cells were evaluated using (B) CCK‐8 assay and (C) colony formation assay. (^** P < .01, compared with HepG2 group or HCC‐LM3 group) (D) Cell migration and(E) invasion assays were performed to test the hypothesis that the overexpression of ACLY may be related to the invasion and migration of HCC cells. (^** P < .01, compared with HepG2‐siNC group). All experiments were repeated 3 times