FIGURE 2.

Suppressing PLCγ1 by U73122 promoted ECM synthesis through autophagy enhancement in IL‐1β‐treated human OA and rat chondrocytes. Cells were treated with IL‐1β (20 ng/mL), U73122 (2 μmol/L) and Chloroquine (50 μmol/L) as described in Figure S1. A&B, The Collagen II, Aggrecan, PLCγ1, p‐PLCγ1, LC3B, P62 and β‐actin protein levels were then detected via Western blotting. C&D, After the immunofluorescence staining was performed, the green immunofluorescence pattern of LC3B and red immunofluorescence pattern of P62 were observed under a laser‐scanning confocal microscope (original magnification × 60). The values represent the means ± SEM of three independent experiments