TABLE 4.

Information of antibodies

Antibody	Manufacturer and Number	Dilution con.	Time
Collagen II	Abcam ab34712	1:100 (IHC)	4℃ over night
Collagen II	Abcam ab188570	1:1000 (WB)	4℃ over night
Aggrecan	Sigma SAB4500662‐100UG	1:100 (IHC) 1:1000 (WB)	4℃ over night
LC3B	NOVUS NB100‐2220	1:200 (IHC) 1:1000 (WB)	4℃ over night
P62 (SQSTM1)	Abcam ab56416	1:500 (IHC) 1:1000 (WB)	4℃ over night
PLCγ1	Cell Signaling technology #2822	1:100 (IHC) 1:1000 (WB)	4℃ over night
p‐PLCγ1 (Tyr783)	Cell Signaling technology #2821	1:100 (IHC) 1:1000 (WB)	4℃ over night
Erk1/2	Cell Signaling technology #4695	1:1000	4℃ over night
p‐Erk1/2 (Thr202/Tyr204)	Cell Signaling technology #9106	1:1000	4℃ over night
Akt	Cell Signaling technology #2920	1:1000	4℃ over night
p‐Akt (Ser473)	Cell Signaling technology #4051	1:1000	4℃ over night
mTOR	Cell Signaling technology #2983	1:1000	4℃ over night
p‐mTOR (Ser2448)	Cell Signaling technology #2971	1:1000	4℃ over night
ULK1	Abcam ab128859	1:1000	4℃ over night
p‐ULK1 (Ser757)	Cell Signaling technology #6888	1:1000	4℃ over night
AMPK	Cell Signaling technology #5831	1:1000	4℃ over night
p‐AMPK (Thr172)	Cell Signaling technology #2535	1:1000	4℃ over night
FAK	Cell Signaling technology #3285	1:1000	4℃ over night
p‐FAK (Tyr397)	Cell Signaling technology #3283	1:1000	4℃ over night
IP3R	Abcam ab5084	1:100 (IP) 1:1000 (WB)	4℃ over night
Bcl‐2	Cell Signaling technology #2870	1:100 (IP) 1:1000 (WB)	4℃ over night
Beclin1	Cell Signaling technology #3738	1:100 (IP) 1:1000 (WB)	4℃ over night
β‐actin	Sigma A3854	1:50 000	RT 20 min
Anti‐Mouse‐IgG (H + L) HRP	Proteintech SA00001‐1	1:25 000	RT 1 h
Anti‐Rabbit‐IgG (H + L) HRP	Proteintech SA00001‐2	1:25 000	RT 1 h
Anti‐Mouse‐IgG (H + L) Alexa Fluor 594	Thermo Fisher Scientific A21203	1:400	RT 4 h
Anti‐Rabbit‐IgG (H + L) Alexa Fluor 488	Thermo Fisher Scientific A21206	1:400	RT 4 h