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. 2020 Dec 28;25(3):1531–1545. doi: 10.1111/jcmm.16245

TABLE 5.

Primers in qPCR

Gene name Primer sequence (5′‐3′)

Col2a1(H)

Gene ID: 1280/NM_033150.3

Forward primer: AGCAGGCGTAGGAAGGTCAT

Reverse primer: AGAACTAATGGAGCAGCAAGA

Col2a1(R)

Gene ID: 25412/NM_012929.1

Forward primer: TCCTAAGGGTGCCAATGGTGA

Reverse primer: GGACCAACTTTGCCTTGAGGAC

ACAN(H)

Gene ID: 176/XM_011521314.1

Forward primer: ACTCTGGGTTTTCGTGACTCT

Reverse primer: ACACTCAGCGAGTTGTCATGG

ACAN(R)

Gene ID: 58968/ J03485.1

Forward primer: TCCGCTGGTCTGATGGACAC

Reverse primer: CCAGATCATCACTACGCAGTCCTC

GAPDH(H)

Gene ID: 2597/NM_001289745.3

Forward primer: ATGGGGAAGGTGAAGGTCG

Reverse primer: TAAAAGCAGCCCTGGTGACC

GAPDH(R)

Gene ID: 24383/XM_017592435.1

Forward primer: CAAGTTCAACGGCACAGTCAAG

Reverse primer: ACATACTCAGCACCAGCATCAC