FIGURE 7.

PD98059 up‐regulates the expression of NOX4 and NLRP3/caspase‐1 pathway. A, Representative Western blots showing the levels of NOX4, P‐ERK1/2, T‐ERK1/2, caspase‐1, NLRP‐3, cleaved caspase‐1, IL‐1β and GAPDH. Semi‐quantification of NOX4 level (B), the P‐ERK1/2 density/T‐ERK1/2 density ratio (C), NLRP3 level (D), the cleaved caspase‐1 density/caspase‐1 density ratio (C) and IL‐1β level (E). The expression levels of target proteins were normalized to those of GAPDH. G. A proposed schematic diagram of the molecular pathway activated by NRG‐1. Data are represented as mean ± standard error of mean (n = 6; *P < .05 vs IR; ##P < .001 vs. NRG‐1). CON, sham‐operated control; IR, ischaemia‐reperfusion; NRG‐1, IR + NRG‐1; PD, PD98059