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. 2021 Feb 10;11:3507. doi: 10.1038/s41598-021-82934-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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D. hansenii growth inhibition assay (a) revealed the activation of Lcm production by S. cyanogenus ΔlanI7 upon introduction of extra copy of lcm cluster-situated transcriptional regulators. Agar plugs were cut from the lawns of S. cyanogenus ΔlanI7 (1), ΔlanI7 lcmRI⁺ (2), ΔlanI7 lcmRII⁺ (3) and ΔlanI7 lcmRIII⁺ (4) grown on YMPG agar for 120 h. Lcm production by the same strains in liquid YMPG was quantified via LC–MS (b) in the three recombinant strains as compared to the initial one. Data represent mean values of three independent experiments, error bars are mean values ± 2SD.