Table 1.
Description of the ORFs discovered in lcm gene cluster.
| lcm | The closest homologue from antibiotic BGCs | AA sequence identity(%)/E-value | Putative product | Putative function in Lcm biosynthesis |
|---|---|---|---|---|
| lcm1 | N/A | – | – | |
| lcm2 | AviE3 (avilamycin BGC) | 61.92/5e−111 | GDP-D-mannose 4,6-dehydratase (COG1089) | Transformation of GDP-D-mannose into GDP-4-keto-6-deoxy-D-mannose |
| lcm3 | FscTE (candicidin BGC) | 60,08/5e−70 | Type II thioesterase (COG3208) | Removal of aberrant intermediates |
| lcmB | PKS modules 2–5 | Lcm aglycone assembly line | ||
| lcmA | PKS initiation and 1 modules | Lcm aglycone assembly line | ||
| lcm4 | AmphDI (amphotericin B BGC) | 58,89/4e−156 | GDP-mycosamine glycosyltransferase (COG1819) | Glycosylation of Lcm aglycone with mycosamine |
| lcm5 | FscMII (candicidin BGC) | 74,43/3e−154 | GDP-3-keto-6-deoxy-D-mannose C-3 aminotransferase (COG0399) | Transformation of GDP-3-keto-6-deoxy-D-mannose into GDP-mycosamine |
| lcm6 | PimG (pimaricin BGC) | 64,78/5e−153 | Cytochrome P450 monooxygenase (COG2124) | Formation of carboxyl group at C-12 |
| lcm7 | RimH (rimocidin BGC) | 66,13/8e−13 | Ferredoxin (COG1141) | Electron transfer in P450 system |
| lcmE | PKS module 12 | Lcm aglycone assembly line | ||
| lcmD | PKS module 11 | Lcm aglycone assembly line | ||
| lcmC | PKS modules 5–10 | Lcm aglycone assembly line | ||
| lcm8 | PimA (pimaricin BGC) | 57,71/3e−147 | ABC transporter (COG1132) | Export of Lcm |
| lcm9 | AmphG (amphotericin B BGC) | 56,20/1e−171 | ABC transporter (COG1132) | Export of Lcm |
| lcm10 | NysL (nystatin BGC) | 42,46/3e−81 | Cytochrome P450 monooxygenase (COG2124) | Epoxidation of Lcm aglycone at C4-C5 |
| lcmRI | NysRIV (nystatin BGC) | 39,38/1e−29 | HTH-LuxR domain containing TF | Regulation of Lcm biosynthesis |
| lcmRII | PimM(pimaricin BGC) | 40,1/2e−32 | HTH-LuxR domain containing TF | Regulation of Lcm biosynthesis |
| lcm11 | PimE | 79,34/0 | Cholesterol oxidase (COG2303) | “Fungal sensor” for ergosterol detection |
| lcmRIII | PimR | 77,9/0 | Transcriptional regulator | Regulation of Lcm biosynthesis |