Table 2.
Primers used in this work.
| Primer | Nucleotide sequence (5′–3′), recognition sites for restriction endonucleases are underlined in primer sequence | Purpose |
|---|---|---|
| iAdpA_F | AAATCTAGACGAGCAACGGAGGTACGGACATGAAACTCTCCGGGCGGCGCC | XNR_4181i cloning |
| iAdpA_R | AAAGAATTCTCACGGACGGCGGGCCCGGTAGG | -//- |
| xnr4181_XbaIup | AAATCTAGAGGGGGGCTTAGTCACATG | XNR_4181tta- cloning |
| xnr4181_EcoRIrp | AAAGAATTCGGAGCTGTCCTCTCTCAGAC | -//- |
| Xnr4181araC_up | AAATCTAGACGAGCAACGGAGGTACGGACATGCCGGAGGAGATCGGCGCCGAC | adpAscydbd/ adpAsghdbd/ adpAscodbd cloning |
| SCLAd_F | AAATCTAGACGAGCAACGGAGGTACGGACATGCCAGAGGAAATCGGGTCGGAC | adpAscladbd cloning |
| SCLAVADPA_R | AAAGAATTCCATGCGACTACCTTATGG | adpAscladbd cloning |
| SCOADPA_R | AAAGAATTCGCCGTCTGCTCACCTCACGG | adpAscodbd cloning |
| SGHADPA_R | AAAGATATCGCCTCCGGCCCCGTCCGGTGT | adpAsghdbd cloning |
| gbc22_F | TTTTCTAGAGGAGGGTCGGAATGGCGGCGCTGG | lcmRI cloning |
| gbc22_R | TTTGAATTCGGGGTTTCAGCCGGACTCG | -//- |
| gbc23_F | TTTTCTAGAGGAGGCACACCGTGATTGACGAAAC | lcmRII cloning |
| gbc23_R | TTTGAATTCTCCGCTCCCTGTCGTCCTC | -//- |
| gbc25_F | TTTTCTAGAGGAGGACCGTCATGCCCGTACCCC | lcmRIII cloning |
| gbc25_R | TTTGAATTCGACCGCGCGAGCGGTGGCTC | -//- |
| amR_F | CGGGGTCTGACGCTCAGTGGA | aac(3)IV amplification |
| amR_R | AGCGTCTGCTCCGCCATTCG | -//- |
| rt_hrdB_fp | GCTGGCCAAGGAGCTCGAC | 278 bp fragment of hrdB (RT-PCR) |
| rt_hrdB_rp | CGTCGAGGGTCTTCGGCTG | -//- |
| L2_F | ACGCCGGTCCCGATCACGTA | 355 bp fragment of lcm2 (RT-PCR) |
| L2_R | TCGGGAAGGTCGCGGAGACT | -//- |
| L6_F | CGAGTCGCGCACCAAAACGC | 312 bp fragment of lcm6 (RT-PCR) |
| L6_R | TAGCGCAGCAGCTCGTCCAC | -//- |
| L8_F | GGTCGGCCGCGATCTGAGGT | 446 bp fragment of lcm8 (RT-PCR) |
| L8_R | CCCTGCGGGTCAGCACGAAC | -//- |
| L11_F | CTGCGCTGCCGGATCGTCTT | 380 bp fragment of lcm11 (RT-PCR) |
| L11_R | TTCGAGGAGGTGCTGCCCCA | -//- |
| LRIII_F | CGTCACGGATCGCGGGCAC | 430 bp fragment of lcmRIII (RT-PCR) |
| LRIII_R | CGTGCTTTCCAGACGTGCGG | -//- |
| lPKS_F | TGTTGCCCGAGAGCCTCACG | 420 bp fragment of lcmC (RT-PCR) |
| lPKS_R | CTACGGCCGCAACCGCCCC | -//- |
Recognition sites of the restriction endonucleases used for the cloning are underlined.