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. 2021 Feb 10;11:3492. doi: 10.1038/s41598-021-82178-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The preparation of the semitransparent amniotic membrane (stAM) as the matrix for cell culture, and the effects of the stAM on inverted microscopy imaging quality impairments. (a–c) The harvest of the stAM and the preparation as the matrix for cell culture in a transwell. (d,e) The spatial relationship of the stAM, bottom of the transwell, cultivated cells, objective lens of the FF-OCT, and light emitted for obtaining images. (f,g) The stAM was semitransparent and reduced light transmission, and both transwells were added to the culture medium. (h,i) The stAM reduced the imaging quality of cultivated cells under a phase-contrast inverted light microscope.