Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 10;11:3492. doi: 10.1038/s41598-021-82178-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The histological, 3D, and 2D cross-sectional FF-OCT images of multilayered cultivated cell sheets. (a) FF-OCT 3D images of DACNs. Yellow arrows indicate the outline surface of DACNs. Red arrows indicate the epithelial type of cells spread at the bottom of DACNs. (b) FF-OCT 2D images of DACNs. Yellow arrows indicate the outline surface of DACNs. (c,d) Hematoxylin and eosin (H&E) staining of cross-sectional DACNs revealed two different cell types, with epithelial cells covering the entire superficial surface of the colonies of neuron-like cells. (e) A 2D cross-sectional FF-OCT images of DACNs demonstrated epithelial-like cells covering the surface of neuron-like cells. (d,e) Green arrows indicated superficial epithelial cells. Red arrows indicated neuron-like cells. (a,b) Scale bar = 100 μm. (c) scale bar = 50 μm. (d,e) scale bar = 25 μm. DACN: directly adherent colonies of neuron-like cells.