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. 2021 Feb 10;11:3492. doi: 10.1038/s41598-021-82178-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Experimental setup of the Mirau-based FF-OCT for cell imaging. LD: 445-nm laser diode; L₁: 40× aspheric lens; DM: dichroic mirror; OL₁ and OL₂: 40× and 20× objective lenses; SCF: single-clad crystal fibre; MMF: multimode fibre (NA: 0.39); OL₃: 20× objective lens (NA: 0.4); PBS: polarising beam splitter; QWP: quarter-wave plate; PZT: piezo-electric transducer; MOL: home-designed Mirau objective lens; LS: transversal linear stage, CG; cover glass; M: mirror; L₂: tube lens (focal length: 150 mm); CCD: charge-coupled device. (b) Schematic illustration of the MOL with OL: 20× water immersion objective lens; GP₁ and GP₂: the first and second glass plates; n_oil: refractive index of silicon oil (n = 1.406); RC: reflection coating; RH: ring holder; B: 420-µm-diameter black absorber spot. (c) Photograph of the Mirau-based FF-OCT. (d) The emission spectrum of Ce³⁺:YAG SCF. (e) and (f) are the axial and lateral resolutions of 0.9 and 0.8 µm, respectively. The inset of (f) is the resolution test target imaged by FF-OCT.