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. 2021 Feb 10;12:907. doi: 10.1038/s41467-021-21242-z

Fig. 6. Silencing of ORAI3 in RA T cells reduces synovial inflammation.

Fig. 6

a, b NSG mice engrafted with human synovial tissue were reconstituted with CD45RO– PBMC from RA patients transfected with control or ORAI3 siRNA. Tissue was collected on day 7 after reconstitution and analyzed. a Three-color immunofluorescence staining of tissue sections for IFN-γ and CD3 (left). Scale bars, 20 µm. Frequencies of CD3+ and IFN-γ+CD3+ T cells in synovial tissues are shown as mean ± SEM of ten random fields of six synovial grafts for each treatment arm (right). Data were analyzed with unpaired two-tailed Student’s t test. b Expression of genes encoding lineage-determining transcription factors and key inflammatory markers as determined by RT-PCR. Results from tissues of mice (n = 6) adoptively transferred with control cells are shown as white bars and circles, and those from mice with ORAI3-silenced cells as gray bars and squares. Data are presented as mean ± SEM and analyzed with unpaired two-tailed Student’s t test. Source data are provided as a Source Data file.