Fig. 6.

The exosomal Rab22a-NeoF1 fusion protein promotes M2 polarization in its recipient macrophages via its binding partner PYK2. a Raw264.7 cells were incubated with the indicated exosomes, and differential expression patterns of the M2-like macrophage markers were presented by a heatmap. b Raw264.7 cells treated with the indicated exosomes and then were subjected to Western blotting. The relative intensity of p-Stat3 and p-Stat5 were quantified with the ImageJ software and normalized with Stat3-Total and Stat5, respectively. Data are representative of n = 3 biologically independent experiments. c, d Mice lungs pre-educated with the indicated exosomes were isolated and analyzed by IHC for expression CD11b (c) and CD206 (d) expression. n = 4 biologically independent mice. Bar plots show CD11b and CD206 staining grade. e–g Mice were pre-educated with the indicated exosomes for 3 weeks, and then were orthotopically injected with 143B-Luc cells. The lung metastases were analyzed after 3 weeks. Representative IVIS imaging (e), H&E-stained lung sections (f), and quantification of lung metastatic foci (g). n = 5 biologically independent mice. Data are mean ± s.d. P values are shown. Scale bar, 2 mm