Fig. 7.

Blockage of the interaction between Rab22a-NeoF1 and PYK2 inhibits the RhoA activation, migration, and invasion of the recipient cells induced by the exosomal Rab22a-NeoF1 fusion protein. a The 293T cells were co-transfected with the indicated plasmids and then were lysed and analyzed by immunoprecipitation using anti-Flag beads followed by Western blotting. b ZOS-M cells were incubated with the indicated peptide for 24 h, and then were lysed and subject to immunoprecipitation using anti-IgG or anti-mAb RAD5–8, followed by Western blotting. WCL whole-cell lysate of ZOS-M cells. c, d ZOS-M cells (c) and the indicated stable 143B cells (d) were treated with the indicated peptide for 24 h, and then the exosomes were purified and analyzed by Western blotting. e U2OS cells were incubated with the indicated exosomes for 1 h and then were lysed and subjected to RhoA activation assay. Data in a–e are representative of n = 3 biologically independent experiments. f U2OS and 143B cells were treated with the indicated exosomes for 24 h, and then were subjected to migration and invasion assays. Data are mean ± s.d. of n = 3 biologically independent experiments. P values are shown