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. 2021 Feb 10;4:179. doi: 10.1038/s42003-021-01699-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b The probability maps of cell nuclei and their membrane were returned from DCNN-cN and overlaid on their corresponding BM4D filtered images. c The initial segmentation of cell nuclei contained topological errors as the membrane of cell nuclei exhibited discontinuity. d We rectified the segmentation of cell nuclei in a GDM framework and excluded non-nucleus instances by an SVM with a quadratic kernel. e 3D rendering of cell nuclei in the contralateral corpus callosum and cingulum of sham #25 dataset.