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. 2021 Feb 10;4:182. doi: 10.1038/s42003-021-01706-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Confocal images show the effect of the miR-96-5p mimic and/or inhibitor transfection on the intensity of CMAC as a marker of GSH (blue) and the expression of GTRAP3-18 (green). Scale bar, 50 μm. Insets in panels are enlarged images corresponding to the box highlighted on the full images. Scale bar, 10 μm. b Quantification of the relative CMAC density in a is shown. Data are mean values ± SD obtained from independent samples and individual data points are plotted (n = 12 for negative control or miR-96-5p mimic, n = 7 for miR-96-5p inhibitor and n = 10 for both miR-96-5p mimic and inhibitor transfection). Data were analyzed by one-way ANOVA (F(3,37) = 14.345, p = 0.0000023) and Tukey’s HSD test. *p < 0.05 relative to the negative control. ^†p < 0.05 versus the effect of the miR-96-5p inhibitor. c The endogenous expressions of GTRAP3-18 and β-actin in Neuro2a cells with transfection of miR-96-5p mimic and/or inhibitor are shown. Molecular weight markers are depicted at right. Full blots are presented in Supplementary Fig. 12. d Quantification of the data in panel c by densitometry. Data represent mean values ± SD obtained from three independent experiments and individual data points are plotted. Data were analyzed by one-way ANOVA (F(3,8)=79.74, p = 0.0000027) and Tukey’s HSD test. *p < 0.05 relative to the negative control. ^†p < 0.05 versus the effect of the miR-96-5p inhibitor. e Relative luciferase activity in SH-SY5Y cells transfected with the luciferase plasmid of GTRAP3-18 3′-UTR with the miR-96-5p mimic and/or inhibitor is shown. Data represent mean values ± SD obtained from three individual experiments and individual data points are plotted. Data were analyzed by one-way ANOVA(F(3,8) = 31.27, p = 0.000091) and Tukey’s HSD test. *p < 0.05 relative to the negative control. ^†p < 0.05 versus the effect of the miR-96-5p inhibitor.