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. 2021 Feb 10;4:182. doi: 10.1038/s42003-021-01706-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a The endogenous expressions of GTRAP3-18, NOVA1 and β-actin after transfection of NOVA1 siRNA (+) or negative control siRNA (−) are shown. Molecular weight markers are depicted at right. Full blots are presented in Supplementary Fig. 15. b Densitometric quantification of the NOVA1 expression in a is shown. Data represent mean values ± SD obtained from six individual experiments and individual data points are plotted. Data were analyzed Student’s t-test, two-sided. *p < 0.05 relative to the negative control. c Densitometric quantification of the GTRAP3-18 expression in a is shown. Data represent mean values ± SD obtained from three individual experiments and individual data points are plotted. Data were analyzed by Student’s t-test, two-sided. *p < 0.05 relative to the negative control. d A schematic plot of the luciferase constructs of GTRAP3-18 3′-UTR is shown. The sequence for the predicted NOVA1-binding site is shown in the box. Mutation was added in a core sequence (red font) of tandem YCAY clusters (bold font). The mutated sequence contains a predicted binding site of Ptbp1 (Ptbp1-mut). e Relative luciferase activities in SH-SY5Y cells transfected with the luciferase plasmids in d with the NOVA1 siRNA or negative control (NC) are shown. Data represent mean values ± SD obtained from independent samples (n = 4 for control, n = 3 for Ptbp1-mut) and individual data points are plotted. Data were analyzed by Student’s t-test, two-sided. *p < 0.05 relative to the negative control.