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. 2021 Feb 10;11:3466. doi: 10.1038/s41598-020-78978-9

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Agent intensity of Cutthroat trout virus (CTV-2; A), Piscine orthoreovirus (PRV; B), Tenacibaculum maritimum (Te_mar; C), and Kudoa thyrsites (Ku_thy; D) in farmed Atlantic salmon throughout four production cycles. Grey circles represent live fish, and black X’s represent dead/dying fish. Curves indicate mean predictions from a generalised additive model; blue and red correspond to live and dead/dying fish, respectively (shaded areas show 95% confidence regions). Left-hand grey region indicates freshwater hatchery residence, grey regions on x-axis indicate period of transfer to another site, and vertical dotted lines correspond to January 1st. Horizontal grey line indicates limit of detection (yielding ≈90% true positive rate) for respective qPCR assay run in duplicate. Note log scale.