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. 2021 Feb 10;11:3516. doi: 10.1038/s41598-021-82883-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Isolation and characterization of primate urinary cells. (a) Workflow of cell isolation from primate urine samples. NC Normocure, REMC renal epithelial mesenchymal cell medium. (b) Primary cells obtained from human, gorilla and orangutan samples are morphologically indistinguishable and display similar EmGFP transduction levels. Scale bars represent 400 μm. (c) The package SingleR was used to correlate the expression profiles from six samples of primate urinary cells (passage 1–3) to a reference set of 38 human cell types. Normalized scores of the eight cell types with the highest correlations are shown (MSC mesenchymal stem cells, SM smooth muscle, Epi epithelial, Endo endothelial). Color bar indicates normalized correlation score. (d) Principal component analysis of primary cells from single colony lysates using the 500 most variable genes. (e) Heatmap of normalized SingleR scores show that cluster C is classified as epithelial cell originating from the bladder. The scores for MSCs in Cluster A and B are similarly high, although cluster B also shows higher scores for epithelial cells than cluster A. See also Supplementary Figure S5.