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. 2021 Feb 10;11:3515. doi: 10.1038/s41598-021-82852-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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A single digital slice (z view) of an electron tomogram of SARS-CoV-2 particles. The ortho-slice view shows the particle labelled by the white cross lines in side view (x/z and y/z) of the volume at the indicated section plane. The particle appears ovoid in shape and the thickest part of the particle in z was selected for size measurement. Note that the section is compressed in z and thinner than the nominal 180 nm set at the microtome, which also affects the shape of the particle viewed in x/z and y/z. This artifact is well known in electron tomography of plastic sections and only slightly affects the size in x/y²³ which our shrinkage measurements demonstrated (see Results). Scale bar = 100 nm.