Figure 3.

LN cellular subsets rapidly induced by immunization correlate with SIV-specific antibody responses at mucosal sites. Correlation between total GC B cells elicited at (A) day 3 post-2^nd adeno and vaginal Env-specific IgG responses at week 16 and (B) day 3 post-2^nd boost and vaginal Env-specific IgG responses at week 41. No correlation was seen between GC B cells elicited at (C) 2 weeks post-adeno and vaginal Env-specific IgG titers at week 16 or (D) GC B cells elicited at 2 weeks post-boost and vaginal Env-specific IgG titers at week 41. (E) LN Env-specific T_FH cells induced at day 3 post-2^nd adeno strongly correlated with Env-specific IgA titers in rectal secretions at week 16. Rectal Env-specific IgA antibody titers at week 41 positively correlated with B cell populations associated with GC maturation including (F) centrocytes and (G) GC Env-specific memory B cells. Correlation analyses were performed using Spearman correlations.