Table 1.

Antibodies used in flow cytometry assays for identification of T_FH cells in LNs, cT_FH cells in PBMCs, and B cells in LN and PBMCs.

Assay	Marker	Clone	Fluorochrome	Company	Staining
AIM assay, LN TFH Cells	CD49d	R1-2	unconjugated	BD Biosciences	Stimulation
	CD28	CD28.2	unconjugated	BD Biosciences	Stimulation
	CCR7 (CD197)	2D12	PerCP-eFluor780	eBioscience	Stimulation
	PD-1	EH12.2H7	BV605	BioLegend	Surface
	CD25	BC96	PE-Cy5	BioLegend	Surface
	CXCR5	MU5UBEE	PerCP-eFluor710	eBioscience	Surface
	CD134	L106	PE	BD Biosciences	Surface
	CD4	L200	BV711	BD Biosciences	Surface
	Live/Dead	–	Aqua Dye	Invitrogen	Surface
	Ki67	B56	BV786	BD Biosciences	ICS
	CD3	SP34-2	AlexaFluor-700	BD Biosciences	ICS
	Bcl6	K112-91	BV421	BD Biosciences	ICS
AIM assay, cTFH cells	CD49d	R1-2	unconjugated	BD Biosciences	Stimulation
	CD28	CD28.2	unconjugated	BD Biosciences	Stimulation
	CCR7 (CD197)	2D12	PerCP-eFluor780	eBioscience	Stimulation
	PD-1	EH12.2H7	BV786	BioLegend	Surface
	CD25	BC96	PE-Cy5	BioLegend	Surface
	CXCR5	MU5UBEE	PerCP-eFluor710	eBioscience	Surface
	CD134	L106	PE	BD Biosciences	Surface
	CD4	L200	BV711	BD Biosciences	Surface
	Live/Dead	–	Aqua Dye	Invitrogen	Surface
	CD14	Tuk4	Qdot605	ThermoFisher	Surface
	CD16	CB16	BV605	eBioscience	Surface
	CD3	SP34-2	AlexaFluor-700	BD Biosciences	ICS
	Bcl6	K112-91	BV421	BD Biosciences	ICS
	Foxp3	206D	Alexa647	BioLegend	ICS
B cells	CD4	19thy5D7	unconjugated	NHP reagent resource	Surface
	CD4	OKT4	unconjugated	NHP reagent resource	Surface
	CD2	S5.5	Qdot605	ThermoFisher	Surface
	CD14	Tuk4	Qdot605	ThermoFisher	Surface
	CD20	2H7	BV650	BioLegend	Surface
	CD27	O323	PerCp-Cy5.5	eBioscience	Surface
	IgD	Polyclonal	Texas red	SouthernBiotech	Surface
	CD21	B-ly4	BV711	BD Biosciences	Surface
	CXCR4	12G5	BV785	BioLegend	Surface
	CD95	DX2	Pe-Cy7	BD Biosciences	Surface
	CD19	J3-119	PE-Cy5	Beckman Coulter	Surface
	CD138	DL-101	PE	BioLegend	Surface
	Ki67	B56	Alexa700	BD Biosciences	ICS
	IRF4	IRF4-3E4	FITC	ThermoFisher	ICS
	BCL6	K112-91	APC-Cy7	BD Biosciences	ICS

ICS, Intracellular Staining.