. 2021 Jan 28;11:608003. doi: 10.3389/fimmu.2020.608003

Table 2.

Immunological correlates between circulating T_FH cell subpopulations and vaccine-induced humoral responses in bone marrow and at mucosal sites.

Vaccine-induced Env-specific responses	cT_FH phenotype	cT_FH functional subpopulation	r value	P value
Bone Marrow IgG-secreting PBs/PCs	CD95⁺ PD-1⁺ cT_FH	Total memory	0.58	0.052^§
	CCR6⁺ PD-1⁺ cT_FH	Th17-like memory	0.72	0.011
	CCR7⁺ PD-1⁺ cT_FH	Central memory	0.67	0.019
Bone Marrow IgA-secreting PBs/PCs	CD95⁺ PD-1⁺ cT_FH	Total memory	0.77	0.005
	CCR6⁺ PD-1⁺ cT_FH	Th17-like memory	0.84	0.001
	CCR7⁺ PD-1⁺ cT_FH	Central memory	0.59	0.047
Vaginal IgG titers	CD25⁺ PD-1⁺ cT_FH	Effector memory	-0.66^#	0.022
	CD95⁺ PD-1⁺ cT_FH	Total memory	-0.66^#	0.024
	CXCR3^- PD-1⁺ cT_FH	Resting memory	-0.69^#	0.015
Rectal IgA titers	Total cT_FH		0.71	0.01
Rectal IgA titers	CD95⁺ PD-1⁺ cT_FH	Total memory	-0.57^#	0.059^§
Rectal IgA titers	Total cT_FH**		0.93	<0.001
	PD-1⁺ cT_FH**	GC-like cT_FH cells	0.65	0.049
	CD95⁺ PD-1⁺ cT_FH**	Total memory	0.65	0.049
	CD25⁺ PD-1⁺ cT_FH**	Effector memory	0.93	0.003
	CCR6⁺ PD-1⁺ cT_FH**	Th17-like memory	0.65	0.049
	CXCR3^- PD-1⁺ cT_FH**	Resting memory	0.66	0.044

All cT_FH frequencies were calculated based on the total CD4⁺ T cell population. Analyses were performed using Spearman’s rank correlation. PBs, plasmablasts; PCs, plasma cells. ^#indicates negative correlations. ^§indicates near significant correlations. Vaccine-induced Env-specific humoral responses in first column were assessed at week 41; cT_FH cell populations in second column were assessed at week 38 + 3 days with exception of cT_FH cell populations indicated by ** which were assessed at week 41.