FIGURE 5.

Screening of putative transformants under kanamycin selection. (A) Transfer of seedlings of independent T₀ plants after kanamycin treatment, (B) cup tray screening for antibiotic resistance, (i) overview of cup tray with planted seeds, (ii) initial signs of kanamycin induced necrosis after 4^th day of treatment, (C) variation in root and shoot lengths of plants after 10^th day of kanamycin treatment, (i) treated wild type, (ii) untreated wild type, (iii,iv) transgenic plants with phenotype at par with untreated wild type, (D) an overview of (i) treated wild type plants exhibiting severe necrosis, (ii) untreated wild type, (iii) putative transgenics, and (E) an overview of the variable response in T₁ seedlings to kanamycin treatment exhibiting resistance and susceptibility to kanamycin.