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. 2021 Jan 28;9:598364. doi: 10.3389/fcell.2021.598364

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Copyright © 2021 Li, Qi, Yao, Zhu, Li, Xu, Zhu, Zhao, Wang, Ding and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The abundance and location of SIRT1 in the endometrium. (A) The representative blot of SIRT1 in endometria from CTRL (n = 6) and RIF (n = 6). (B,C) The abundance of SIRT1 in endometria from CTRL (n = 12) and RIF (n = 12). The SIRT1 expression levels were normalized to β-actin. (D) Immunohistochemical staining of SIRT1 in human uterine endometrium (WOI phrase), the negative control (NC) showed no brown staining. EEC, endometrial epithelial cells; ESC, endometrial stromal cells. (E) The mRNA levels of IGFBP1, PRL, DIO2, and SCARA5 in endometria from CTRL (n = 12) and RIF (n = 12). (F) Correlation analysis of SIRT1 and PRL mRNA levels in the endometrium from CTRL group and RIF group. (G) Correlation analysis of SIRT1 and SCARA5 mRNA levels in the endometrium from CTRL group and RIF group. ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001 (data are means ± SEM).